Occupational hazards are very common in health care setting. They are common in any area of work, including hospitals. Clients in all health care setting are at risk for acquiring infection because of exposure to number and types of diseases. Sharp used in hospitals include syringe, needle, scalpels, broken glass and other objects contaminated with blood form a source of infection. Due to NSIs, the risk of infections ranges from as low as 0.2–0.5% for HIV to as high as 3–10% for HCV and 40% for HBV. The purpose of this study is to examine awareness about the needle stick and splash related injuries among the health care workers in public health hospitals from June 2016 to December 2016, and classify the frequencies of those cases by job categories and location of injury. We will also study the effect of certain variables such wearing protective equipment, job categories and location of occurrence, type of Injury. We will also study the prevalence and associated factors of Needle Stick Injuries (NSI) among the health care workers of our hospital also To assess the level of awareness of the health care workers regarding NSI. Data will obtain from health care workers in public health hospitals in Indore, India from January 2017 to June 2017. A cross-sectional descriptive Study is plan and data was collected by using a pretested close ended structured questionnaire among the respondents of the health care workers of a tertiary care public hospital. We have used mean, Standard deviation (SD), p value and Z test and other statistical tools. We have taken z test as a test of significance with fixing the p value of 0.05 as significant.