Proposed work was conducted to screen and evaluate the activity of the n-Hexane extract of Sonchus asper against anemia by analyzing its effects on Red Blood Cells (RBC), Hemoglobin (HgB) and Hematocrit (HCT) level of blood in Sprague Dawley rats, which were induced anemia using phenylhydrazine. Collection and authentication of Sonchus asper were done followed by extraction with Soxhlet extractor using n-Hexane as a solvent. A phytochemical investigation was carried out using a standard protocol. Anemia was induced in Sprague Dawley rats by a single Phenylhydrazine (i.p.) administration at a dose of 40mg/kg. Sonchus asper n-Hexane extract was administered to test group and standard marketed formulation, Folic acid Tablets (Fol*-5) was given to the standard group of animals. Evaluation of blood profile was carried out on the 14th day followed by a comparison between test, control and standard groups of animals for their red blood cells, Hemoglobin, hematocrit level of blood. The data obtained from this work suggests that Sonchus asper n-Hexane extract shows a significant increase (p<0.05) in red blood cells, hemoglobin and hematocrit level of blood in Sprague Dawley rats. The Sonchus asper n-Hexane extract shows the most promising activity as compared to standard formulation. This study concludes that Sonchus asper n-Hexane extract shows antianemic activity hence it acts as a potent therapeutic agent against anemia as it shows hepatoprotective potential.