This paper is published in Volume 3, Issue 4, 2018
Area
Analytical Method Development
Author
Dr. Anantharama. G Nayaka
Co-authors
Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh
Org/Univ
Central Drugs Testing Laboratory, Mumbai, Maharashtra, India
Keywords
RP-HPLC, Ciprofloxacin Hydrochloride, Betamethasone Sodium Phosphate, Simultaneous and Ceflox-D Eye/ear Drops.
Citations
IEEE
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh. RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARnD.com.
APA
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh (2018). RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms. International Journal of Advance Research, Ideas and Innovations in Technology, 3(4) www.IJARnD.com.
MLA
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh. "RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms." International Journal of Advance Research, Ideas and Innovations in Technology 3.4 (2018). www.IJARnD.com.
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh. RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARnD.com.
APA
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh (2018). RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms. International Journal of Advance Research, Ideas and Innovations in Technology, 3(4) www.IJARnD.com.
MLA
Dr. Anantharama. G Nayaka, Arun. K Nagarkar, Dr. Vijay Kumar M, Manasi Patel, Dr. Raman Mohan Singh. "RP-HPLC method development and validation for the Estimation of Ciprofloxacin HCl and Betamethasone Sodium Phosphate in Eye/Ear drop dosage forms." International Journal of Advance Research, Ideas and Innovations in Technology 3.4 (2018). www.IJARnD.com.
Abstract
A simple precise, accurate Reverse Phase isocratic High-performance liquid chromatography method has been developed for simultaneous determination of Ciprofloxacin Hydrochloride (CIPRO) and Betamethasone Sodium Phosphate (BETA) in Eye/Ear drop Pharmaceutical dosage form.Both drugs are separated by using Purosphere Star C‐18 column (250mm X4.6mm ID 5μm,) with the mobile phase consisting of 50 mM phosphate buffer solution pH 3.0: Acetonitrile (70:30 % v/v), with the flow rate of 1.0mL/min and detection wavelength set at 240nm.The optimum separation was achieved in less than 10 minutes. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. The linearity study of the developed method carried out from the concentration range of 600 to 1800μg/ml for Ciprofloxacin and 20 to 60μg/ml for Betamethasone sodium phosphate, the Correlation coefficient found to be more than 0.999 for both Ciprofloxacin and Betamethasone Sodium Phosphate. The percentage recovery was found 99.41% for Ciprofloxacin and 99.69% for Betamethasone Sodium Phosphate. The method was validated as per ICH guidelines and is suitable for the routine analysis in dosage forms.