The conventional piano is often expensive and not portable, its ambidexterity and ubiquity have made it one of the world’s most familiar and famous musical instruments. Here in this paper, Proposing Implementation of Automatic Piano Player by using MATLAB GUI software. In this paper, we had used the most powerful features of the MATLAB i. e GUI (Graphical User Interface) Toolbox to generate various frequency tones of a piano. This paper presents how MATLAB generates virtual keyboard of a piano via GUI then click on the key using a mouse then it will generate that tone. And depending upon which key has pressed the frequency related that key will be played.