Colon targeted drug delivery system is capable of protecting the drug in route to the colon i.e. drug release and absorption does not occur in the stomach and small intestine but only released and absorbed once it reaches the colon. Fenoprofen tablets were prepared by wet granulation Technique using different ratios of Fenoprofen with polymers like dikamali gum, karaya gum, gum kondagogu, okra gum, and Eudragit RL100. In the present study, the sustained release tablets were prepared with hydrophilic polymers like gum dikamali along with other natural polymers. Gum dikamali is Gardenia gummifera belonging to the family Rubiaceae, are medium-sized trees growing all over India. The gum-resin oozing out from the leaf buds of these trees is called Dikamali gum. The natural polymer selected for the present study was Dikamali which is a hydrophilic matrix forming agent. Eudragit RL 100 was used as a polymer for targeting the drug to the colon. The in-vitro drug release studies showed that the drug release was sustained in a better way in the colon for 24hours with dikamali gum in combination with other natural polymers. In-vitro dissolution studies of fenoprofen tablets revealed that the formulation F19 containing dikamali gum as a polymer shows maximum drug release at the end of 24hours when compared with the other formulations. Drug release kinetics of the optimized formulation states that the formulation F19 follows zero order drug release with fickian diffusion mechanism.