Dental fear is a normal emotional reaction to one or more specific threatening stimuli in the dental situation. Fear of pain is an important issue in the practice of dentistry because it interferes with both, the provision and the receipt of dental care. The present study was conducted to assess fear of dental pain among students of dental colleges in Lucknow city. A cross-sectional study was undertaken to assess the fear of dental pain among undergraduate dental students. A self administered questionnaire was used to obtain the data. The questionnaire consisted of two parts. Part one consisted of basic demographic data of the subjects. Part two was a modified version of fear of dental pain (FDP) questionnaire.6 Fear of dental pain questionnaire is a self-administered questionnaire measuring pain-related fear to a variety of painful stimuli during routine dental treatment. It consisted of a set of 18 questions related to routine dental treatment. Among 1050 subjects majority 680(64.8%) were females and 370(35.2) were males. The highest score was for fear of being drilled in the jaw bone (2.11±1.24) and lowest was for fear to get the filling of molar teeth done (0.94±1.13). The fear score was observed to be higher among male (1.70±0.74) students compared with females (1.44±0.55) and the difference was significant (p=0.0001). The post-doc comparison tests revealed that the fear score was significantly higher among the students of 1st year (1.64±0.68). Dental fear is a major concern that should be dealt with and managed. Dental students, especially in 1st year had more fear than 4th year students. Male dental students had more fear than female dental students. “Exposure therapy” is recommended for the junior students for the exposure of feared objects to reduce the fear gap between the students of different years.