Carissa carandas (C. carandas) fruits are well known ancient natural medicine owning high antioxidant and antidiabetic activity. It is a straggly, woody, climbing shrub rich in white gummy latex with sharp thorns and belonging to the family Apocynaceae. Because of its eminent medicinal property, the present study was conducted to investigate the antioxidant and antidiabetic properties of C. carandas fruit along with DNA damage inhibition assay. C. carandas type 1 (T1) and type 4 (T4) fruits extract were prepared in methanol. In vitro, antioxidant activity was evaluated by four vitro assays viz. DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay, FRAP (Ferric Reducing Antioxidant Power) assay and ABTS (2, 2’-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) radical cation depolarization assay. Further anti-diabetic activity was also investigated by performing Alpha-Glucosidase Inhibitory Assay. The extracts scavenged DPPH radicals efficiently. The scavenging effect was observed in case of T1 extract little higher than T4 extract. FRAP scavenging activity and also ABTS assay for methanolic extract of C. carandas were also analyzed, where result showed to be an activity of T1 was observed more than T4. The effect of C. carandas fruit extract on the activities of α-amylase and β–glucosidase were evaluated to determine anti-diabetic activity. The T1 fruit extract was shown formidable inhibition of β–glucosidase and alpha-amylase activity over T4. Green and pink colored fruit extracts were used in DNA damage inhibition assay, resulted in green colored fruit has more antioxidant activity over pink and showed potent inhibition of DNA damage.