Separation of Metformin and Saxagliptin was successfully achieved thermo, C18 250X4.6mm, 5µm, or equivalent in an isocratic mode utilizing 0.1M KH2PO4: Methanol (65:35) at a flow rate of 1.0ml/min and elute was monitored at 256nm, with a retention time of 2.787 and 3.436 minutes for Metformin and Saxagliptin respectively. The method was validated and their response was found to be linear in the drug concentration range of 50µg/ml to 150µg/ml for Metformin and 50µg/ml to 150µg/ml for Saxagliptin. The LOD and LOQ for Metformin were to be 0.747 and 2.488 respectively. The LOD and LOQ for Saxagliptin were found to be 0.0268 and 0.0893 respectively. This method was found to be a good percentage recovery for were found to be 99.76% and 99.77% respectively indicates that the proposed method is highly accurate. The method was extensively validated according to ICH guidelines for Linearity, Accuracy, Precession, Specificity, and Robustness.